Goat Anti-Rabbit IgG (H&L) Highly Cross-Adsorbed Antibody (AcalephFluor594)

The Goat Rabbit IgG (H&L) Highly Cross-Adsorbed - AcalephFluor594 Antibody is suitable for ELISA, FC, IF, IHC. It is a Polyclonal, AcalephFluor594 conjugated antibody which raised against Rabbit IgG. Purification: Goat Polyclonal Secondary Antibody to Rabbit IgG (H&L) have been cross-adsorbed against IgG from bovine, goat, guinea pig, hamster, horse, mouse, rat, and human. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.