Anti-NKX3.1 (NT) Antibody

Rabbit Polyclonal Antibody specific to NKX3.1 Antibody (NT)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Homeobox protein NK-3 homolog A

Gene Name

NKX3-1

NCBI Gene ID

UniProt

Q99801

Cellular Locus

Nucleus

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic peptide corresponding to aa residues 21-37 (TPSKPLTSFLIQDILRD) of human NKX3.1 conjugated to KLH at the C- terminus.

Target Antigen

Homeobox protein Nkx-3.1

Target

NKX3.1 (NT)

Clonality

Polyclonal

Type

Antibody

Applications

WB, ELISA

Field of Research

Cancer research

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Acts as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells. {PubMed:19462257}.

Additionnal Information

Immunoblotting : use at 1:1,000 <br><br>Positive control: Human prostate tissue extracts.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Specificity

This antibody recognizes human NKX3.1 (approx. 26 kDa). This sequence is highly conserved in rat and mouse.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

NKX3.1 is a homeobox gene which exhibits prostate- and testis-specific expression. Loss of NKX3.1 expression has been implicated in prostate development and tumorigenesis. NKX3.1 expression is dramatically down-regulated in testicular cancer of germ cell origin. Results suggest that NKX3.1 is essential for normal testis function and that its loss of expression is highly associated with the invasive phenotype of testicular germ cell tumors.