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PNA-R-PE, Conjugated

Peanut lectin (PNA) is an identical tetrameric carbohydrate free protein with MW of 110 kDa. Thomsen-Friedenreich antigen, T-antigen (Galβ1, 3GalNAc) is present on blood group M &N glycoproteins (after removal of sialic acid with neuraminidase), glyconjugates (Mucin type), gangliosides and many glycolipids. T antigen is rarely expressed on normal coloncytes whereas cells of malignant, premalignant cells express this antigen. Peanut lectin has widely been used to detect T antigen in malignant and premalignant cells. Peanut lectin contains one atom of Ca++ and Mg++ per unit.

Product Specifications

Background

Peanut lectin (PNA) is an identical tetrameric carbohydrate free protein with MW of 110 kDa. Thomsen-Friedenreich antigen, T-antigen (Galβ1, 3GalNAc) is present on blood group M &N glycoproteins (after removal of sialic acid with neuraminidase), glyconjugates (Mucin type), gangliosides and many glycolipids. T antigen is rarely expressed on normal coloncytes whereas cells of malignant, premalignant cells express this antigen. Peanut lectin has widely been used to detect T antigen in malignant and premalignant cells. Peanut lectin contains one atom of Ca++ and Mg++ per unit. Phycoethyrin: Phycoethyrin (R-PE) is a 240 kDa protein from the light-harvesting phycobilliprotein, phycoerythrin is composed of a protein part, organised in a hexameric structure of alpha and beta chains, covalently binding chromophores called phycobilins. In the phycoerythrin family, the phycobilins are: phycoerythrobilin, the typical phycoerythrin acceptor chromophore, and sometimes phycourobilin (marine organisms) . Phycoerythrins are the phycobilliprotein which bind the highest number of phycobilins (up to six per alpha-beta subunit dimer) .

Certification

RUO

Other Statements

For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY

Label

ICT

Type

Lectins

Applications

Flow Cytometry

Concentration

1 mg of PNA conjugated with R-PE

Buffer

10 bicarbonate, 150 mM NaCl, pH 8.2, 0.1 mM Calcium chloride and 0.05% sodium azide

Shipping Conditions

Ships overnight (domestic), International Priority Shipping

Storage Conditions

2-8 °C

Storage Temperature

2-8°C

Target Description

Peanut lectin (PNA) is an identical tetrameric carbohydrate free protein with MW of 110 kDa. Thomsen-Friedenreich antigen, T-antigen (Galβ1, 3GalNAc) is present on blood group M &N glycoproteins (after removal of sialic acid with neuraminidase), glyconjugates (Mucin type), gangliosides and many glycolipids. T antigen is rarely expressed on normal coloncytes whereas cells of malignant, premalignant cells express this antigen. Peanut lectin has widely been used to detect T antigen in malignant and premalignant cells. Peanut lectin contains one atom of Ca++ and Mg++ per unit. Phycoethyrin: Phycoethyrin (R-PE) is a 240 kDa protein from the light-harvesting phycobilliprotein, phycoerythrin is composed of a protein part, organised in a hexameric structure of alpha and beta chains, covalently binding chromophores called phycobilins. In the phycoerythrin family, the phycobilins are: phycoerythrobilin, the typical phycoerythrin acceptor chromophore, and sometimes phycourobilin (marine organisms). Phycoerythrins are the phycobilliprotein which bind the highest number of phycobilins (up to six per alpha-beta subunit dimer).

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