Tween 20

Non ionic detergents like Tween 20 are used in immunoassays for diluting antibodies to lower background, and non-specific antigen-antibody (ag-ab) reaction.

Product Specifications

Other Statements

For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY

Label

ICT

Type

IHC Reagents

Applications

IHC, ELISA

Assay Protocol

For diluting antibodies use 1:1000 in buffers (1 ml into 999 ml). Washing buffer use 1:500 (1 ml into 499 ml). The optimum dilution should be determined by the individual lab. (NOT RECOMMENDED FOR DILUTION OF S100 ANTIBODIES)

Buffer

This detergent is supplied as 50% in water with 0.15 % preservative.

Shipping Conditions

Ships overnight (domestic), International Priority Shipping

Storage Temperature

Room temperature

Target Description

Non ionic detergents like Tween 20 are used in immunoassays for diluting antibodies to lower background, and non-specific antigen-antibody (ag-ab) reaction. This detergent is also used in washing buffer from 0.1- 0.5% in all immunoassays (ELISA, Immunohistochemistry (IHC), Blot assays and others) to reduce background. Tween 20 or Polysorbate 20 PEG(20) sorbitan monolaurate, lowers surface tension of water.