Streptavidin Conjugated with Alkaline Phosphatase

Product Specifications

Other Statements

For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY

Label

ICT

Type

IHC Reagents

Applications

WB, IHC, ELISA

Dilution

1:200-1:1,000 (ICC) ; 1:1,000- 1:10,000 (WB)

Buffer

The complex is supplied in one ml of 10 mM Tris-HCl, 200 mM NaCl, 50% glycerol, pH8.0 buffer containing 10 mg of BSA and 0.05% sodium azide

Shipping Conditions

Ships overnight (domestic), International Priority Shipping

Storage Temperature

2-8 °C; Do not freeze

Target Description

Streptavidin is a 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0; Purity: > 99% pure by HPLC; Eletrophoretically homogenous, single band Protein content: > 95%; Activity 14.8 units/ mg, one unit bunds 1.0 microgram of d-biotin; Protease activity: 0.000018 units/mg; Isoelectric point: 7.0 +/- determined by Isoelectric focusing. Alkaline Phosphatase (ALP) used in this conjugation is 140 KDa enzyme purified from calf intestine.