Streptavidin Conjugated with Fluorescein (FITC)

Streptavidin is a 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC) . Extinction Coefficient 1% A280=32.0. Purity: > 99% pure by HPLC; Eletrophoretically homogenous, single band. Protein content: > 95%; Activity 14.8 units/ mg, one unit bunds 1.0 microgram of d-biotin; Protease activity: 0.000018 units/mg Isoelectric point: 7.0 +/- determined by Isoelectric focusing. One mg of Streptavidin is conjugated with FITC, unconjugated Streptavidin is removed. This conjugate of streptavidin is recommended for use with Biotin~conjugated antibodies. Avidin~Biotin conjugated reagents are stable, sensitive and give less background.

One mg of streptavidin is conjugated with Fluorescein isothiocyanate (FITC) in one ml of 10 mM PO4, 150 mM NaCl, pH 7.4, BSA 10 mg/ml and 0.05% sodium azide

Streptavidin is a 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0. Purity: > 99% pure by HPLC; Eletrophoretically homogenous, single band. Protein content: > 95%; Activity 14.8 units/ mg, one unit bunds 1.0 microgram of d-biotin; Protease activity: 0.000018 units/mg Isoelectric point: 7.0 +/- determined by Isoelectric focusing. One mg of Streptavidin is conjugated with FITC, unconjugated Streptavidin is removed. This conjugate of streptavidin is recommended for use with Biotin~conjugated antibodies. Avidin~Biotin conjugated reagents are stable, sensitive and give less background.