Streptavidin, Unconjugated - Bader says not IHC Reagent?
Streptavidin is a 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC).
Product Specifications
Other Statements
For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY
Label
ICT
Type
IHC Reagents
Applications
IHC, ELISA
Assay Protocol
For conjugation, sodium azide should be removed by dialysis or gel filtration column.
Shipping Conditions
Ships overnight (domestic), International Priority Shipping
Storage Temperature
2-8°C
Target Description
Streptavidin is a 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0.
Purity: > 99% pure by HPLC; Eletrophoretically homogenous, single band.
Protein content: > 95%; Activity 14.8 units/ mg, one unit binds 1.0 microgram of d-biotin;
Protease activity: 0.000018 units/mg
Isoelectric point: 7.0 +/- determined by Isoelectric focusing.
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