Anti-HDEL Antibody
Mouse Monoclonal Antibody specific to HDEL
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Anti-HDEL Mouse Monoclonal Antibody
Host
Mouse
Reactivity
Drosophila, Yeast, Plants
Immunogen
Synthetic peptide corresponding to the C-terminus of yeast Bip.
Target
HDEL
Clonality
Monoclonal
Isotype
IgG2b
Type
Antibody
Applications
WB, ICC/IF
Field of Research
Heat Shock& Stress Proteins
Purification Method
Purified by Protein G affinity chromatography
Concentration
Lot Specific
Dilution
Dilute in PBS or medium which is identical to that used in the assay system.
Format
Purified
Form
Liquid
Buffer
Phosphate Buffered Saline
Additionnal Information
Immunoblotting: use at 0.5-1ug/ml. A band of ~70 kDa is detected.
<br><br>Immunofluorescence: use at 0.2-2ug/ml.
<br><br>These are recommended concentrations. User should determine optimal concentrations for their application.
<br><br>Positive control: S. cerevisiae lysate.
Storage Conditions
This antibody is stable for at least one (1) year at -20°C.
Specificity
This antibody recognizes a ~70 kDa protein corresponding to the predicted molecular mass of HDEL in Drosophila, yeast, and various plants (barnyard grass, beet, cotton, mung bean, sorghum, and wheat).
Formulation
PBS, pH 7.4, 50% glycerol, and 0.09% sodium azide.
Buffer pH
pH 7.4
Target Background
The Hsp70 family includes four highly conserved proteins (Hsp70, Hsc70, Grp75, and Grp78) which function as molecular chaperones, in translocation of polypeptides across cell membranes and to cell nuclei, and in proper folding of nascent polypeptide chains. Sorting of proteins by the Hsp70 family relies on a C-terminal tetrapeptide signal, usually KDEL in animal cells, and HDEL in S. cerevisiae.
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