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Alkaline Phosphatase Enhancer

Alkaline Phosphatase (alk. phos.), an enzyme derived from bovine intestine mucosa is used as a label in situ hybridization and Immunohistochemistry (IHC) .

Product Specifications

Background

Alkaline Phosphatase (alk. phos.), an enzyme derived from bovine intestine mucosa is used as a label in situ hybridization and Immunohistochemistry (IHC) .Several insoluble chromogens are used to detect this enzyme. This stable solution is used to increase the signal generated by alkaline phosphatase. It can be used with Fast red, BCIP/INT, BCIP/NBT, our Fast Red Super (organic solvents stable, cat. # AR-8211) or any other alk phos. chromogen. A single treatment of this reagent is applied before the chromogen.

Certification

RUO

Other Statements

For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY

Label

ICT

Type

IHC Reagents

Applications

IHC, ICC, ISH

Assay Protocol

1. After the alkaline phosphatase reaction, wash slide with buffer, and distilled/ deionized water. 2. Add few drops of this ready to use Enhancing Reagent to cover the tissue/cell smear, for 1-2 minutes and discard. DO NOT WASH SLIDE. 3. Add few drops of ready to use alkaline phosphatase chromogen, follow instructions as outlined for these chromogens.

Shipping Conditions

Ships overnight (domestic), International Priority Shipping

Storage Conditions

Room Temperature

Storage Temperature

Room temperature

Target Description

Alkaline Phosphatase (alk. phos.), an enzyme derived from bovine intestine mucosa is used as a label in situ hybridization and Immunohistochemistry (IHC).Several insoluble chromogens are used to detect this enzyme. This stable solution is used to increase the signal generated by alkaline phosphatase. It can be used with Fast red, BCIP/INT, BCIP/NBT, our Fast Red Super (organic solvents stable, cat. # AR-8211) or any other alk phos. chromogen. A single treatment of this reagent is applied before the chromogen.

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