Peroxidase Signal Enhancer Kit

Peroxidase enzyme system is routinely used in Immunohistochemistry (IHC) and Immunocytochemistry (ICC) . This Peroxidase Enhancing system increases the level of sensitivity in peroxidase based chromogens (AEC and DAB) . This product is used prior to addition of chromogens. We recommend our aqueous mounting medium (ImmunoHistoMount (IHM), cat # AR-6503) for mounting of AEC (counterstain hematoxylin) . For DAB (counterstain hematoxylin) either aqueous mounting medium (IHM, AR-6503) or organic mounting medium (Organo/limonene mounting medium, AR-6504) or equivalent can be used

Mixing of reagent: To five ml buffer (reagent B) in a test tube, add one drop of concentrated Enhancing reagent (reagent E) mix well. This ready to use reagent is good for 4-6 hours. The unused solution can be discarded according to city, county, state, province or country’s regulations. Application: IHC, ICC 1. After the peroxidase reaction, wash slide with buffer 5-7X, (this buffer should not contain any sodium azide because peroxidase is inactivated) and distilled/ deionized water 2X. 2. Add few drops of ready to use enhancing reagent for 1-2 minutes and discard. Washing of slide is not required. 3. Add few drops of ready to use AEC reagent, or DAB reagent, follow instructions as outlined for these chromogens.

Peroxidase enzyme system is routinely used in Immunohistochemistry (IHC) and Immunocytochemistry (ICC). This Peroxidase Enhancing system increases the level of sensitivity in peroxidase based chromogens (AEC and DAB). This product is used prior to addition of chromogens. We recommend our aqueous mounting medium (ImmunoHistoMount (IHM), cat # AR-6503) for mounting of AEC (counterstain hematoxylin). For DAB (counterstain hematoxylin) either aqueous mounting medium (IHM, AR-6503) or organic mounting medium (Organo/limonene mounting medium, AR-6504) or equivalent can be used