EDTA Buffer, 10X

This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.

Product Specifications

Specifications

Antigen Retriever Solution, 10X

Other Statements

For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY

Label

ICT

Type

IHC Reagents

Applications

IHC

Assay Protocol

1. Deparafinize FFPE tissue section is carried out as outlined in your protocol. Hydrate tissue with PBS or our Universal Immuno buffer. 2. The tissue slides are immersed in this 1X solution and heated for 10-20 minutes at 100ºC; OR in pressure cooker for 3-5 minutes followed by cooling at room temperature for about 20 minutes. 3. The tissue sections are ready for further IHC protocol.

Concentration

10X

pH

pH 8.5

Shipping Conditions

Ships overnight (domestic), International Priority Shipping

Storage Temperature

2-8 °C; Do not freeze

Target Description

This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.