Pepsin Reagent (ready to use)

Pepsin is an antigen retriever solution used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.

Product Specifications

Specifications

Ready-to use Pepsin reagent

Other Statements

For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY

Label

ICT

Type

IHC Reagents

Applications

IHC

Assay Protocol

1. Deparafinize FFPE tissue section is carried out as outlined in your protocol. Hydrate tissue with PBS or ImmunoBioScience Universal Immuno buffer. 2. Remove buffer, add 2-4 drops of Pepsin reagent to cover tissue section. 3. Incubate at 37oC or at 56oC for 5-10 minutes (the time required for optimum digestion of FFPE tissue section will vary with the extent of fixation, generally approx. 5-10 minutes are sufficient at the above temperatures. In some cases it may require 15 minute.). 4. Discard Pepsin reagent, wash 3-5 times with buffer. 5. The tissue sections are ready for further IHC protocol. NOT RECOMMENDED FOR CYTOKERATIN (clone: AE3) ANTIBODY

Shipping Conditions

Ships overnight (domestic), International Priority Shipping

Storage Temperature

2-8 °C; Do not freeze

Target Description

Pepsin is an antigen retriever solution used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Pepsin digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens.