Methyl Green

This methyl green counterstain solution is designed for nuclear counterstain after completion of Immunohistochemistry staining or for routine histology. Nuclei will be stained green color, which will provide excellent contrast to brown (DAB, cat. # AR-8202), blue black color (DAB/Cobalt cat. # AR-8204), dark brown / black/blue (DAB/Nickel, cat. # AR-8302) or red (Fast Red Super, Organic solvent stable, cat. # AR-8211) .These chromogens with Methyl Green has to be dehydrated with organic solvents and mounted with Organic mounting medium with our Organo Mount (AR-6504) or any other mounting medium. Aqueous mounting mediums are not compatible with Methyl Green.

A. Immunohistochemistry: 1. After chromogen staining, wash slide 2-3X with de/di water, 2X with buffer and 2X with de/di. water 2. Apply Methyl Green counterstain for 3-5 minutes or longer (if deep green color is required, slide may be incubated at 60ºC for 2-5 minutes) . 3. DO NOT rinse with water. Go directly into 95 % alcohol for few dips and 100% alcohol 5-7X. 4. Clear with Xylene or clearing agent. 5. The slides can be mounted with Organic mounting mediums like Organo mount (limonene) or equivalent. B. Histology: A. Follow the procedure as outlined for hitology 1. Deparafinize formalin-fixed paraffin-embedded (FFPE) tissue sections. 2. At the counterstain step, apply Methyl Green counterstain for 3-5 minutes or longer (if deep green color is required, slide may be incubated at 60ºC for 2-5 minutes) . 3. DO NOT rinse with water. Go directly into 95 % alcohol for few dips and 100% alcohol 5-7X. 4. Clear with Xylene or clearing agent. 5. The slides can be mounted with Organic mounting mediums like Organo mount (ImmunoBioscience cat. # AR-6504) or equivalent.

This methyl green counterstain solution is designed for nuclear counterstain after completion of Immunohistochemistry staining or for routine histology. Nuclei will be stained green color, which will provide excellent contrast to brown (DAB, cat. # AR-8202), blue black color (DAB/Cobalt cat. # AR-8204), dark brown / black/blue (DAB/Nickel, cat. # AR-8302) or red (Fast Red Super, Organic solvent stable, cat. # AR-8211).These chromogens with Methyl Green has to be dehydrated with organic solvents and mounted with Organic mounting medium with our Organo Mount (AR-6504) or any other mounting medium. Aqueous mounting mediums are not compatible with Methyl Green.