Immuno Fluoro Mount with DABCO™

Immuno Fluoro mount with antifading agent 1, 4-Diazobicyclo- (2,2,2-octane (DABCO™) is an aqueous non- permanent, low viscosity mounting medium for immunofluorescence. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine and Redox. The fluorescence is retained during storage at 4oC in the dark. Not recommended for phycoethyrin (PE), phycocyanin (PC) and allophycocyanin (APC) . This medium does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes.

1. Bring the vial to room temperature. 2. Rinse slide to be mounted with DISTILLED OR DEIONIZED WATER, touch the edges of slide on a paper towel to remove excess water. Place slides on a flat surface. 3. Turn the vial upside down and open the dropper to remove any air bubbles. Apply 2-3 drops of mounting medium and apply coverslip carefully without getting air bubbles 4. The specimen is ready for visualization under a microscope. 5. The coverslip may move, so it may be necessary to seal the edges of cover slip with nail polish, any organic medium or our Organo mounting medium (cat. # AR-6504) . 6. Method for applying Coverslip: Put 1-2 drops of Mounting medium on the specimen. After 3-4 minutes apply coverslip carefully avoiding air bubbles. Put Kimwipes on the top of coverslip, press gently to remove excess mounting medium. With 200 micropipette add organic mounting medium to seal the edges. Incubate at 37C for one hour in the dark to dry organic mounting medium. 7. Store slide at 4ºC in the dark.

Immuno Fluoro mount with antifading agent 1, 4-Diazobicyclo-(2,2,2-octane (DABCO™) is an aqueous non- permanent, low viscosity mounting medium for immunofluorescence. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine and Redox. The fluorescence is retained during storage at 4oC in the dark. Not recommended for phycoethyrin (PE), phycocyanin (PC) and allophycocyanin (APC). This medium does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes.