Glycerol mounting medium with DABCO

Glycerol with antifading agent 1, 4-Diazobicyclo-(2,2,2-octane (DABCO™) is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

Product Specifications

Specifications

Ready to use mounting medium with anti-fading agent

Other Statements

For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY

Label

ICT

Type

IHC Reagents

Applications

IHC

Detection Method

Immunofluorescence, confocal microscopy

Assay Protocol

Dehydration of Specimen is not required. 1. Bring the vial to room temperature. 2. Turn the vial upside down and open the dropper to remove any air bubbles. 3. Apply 1-2 drops of mounting medium directly; cover slip carefully avoiding air bubbles. 4. The specimen is ready for visualization under a microscope. 5. One can seal the edges of cover slip with nail polish, any organic medium or our Organo mounting medium (cat. # AR-6504). 6. Method for applying Coverslip: Put 1-2 drops of Mounting medium on the specimen. After 3-4 minutes apply coverslip carefully avoiding air bubbles. Put Kimwipes on the top of coverslip, press gently to remove excess mounting medium. With 200 micropipette add organic mounting medium to seal the edges. 7. For storage it is recommended that the slide be stored in the dark at 2-8oC.

Shipping Conditions

Ships overnight (domestic), International Priority Shipping

Storage Temperature

Room temperature; Long term storage 2-8 °C recommended; Protect from light; DO NOT FREEZE

Target Description

Glycerol with antifading agent 1, 4-Diazobicyclo-(2,2,2-octane (DABCO™) is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine, Redox. Fluorescence is retained during prolonged storage at 4oC in the dark Not recommended for phycoethyrin (PE), phycocyanin (PC) and allophycocyanin (APC)