Fluoroshield with DAPI & DABCO

1. Bring the vial to room temperature. 2. Rinse slide to be mounted with DISTILLED OR DEIONIZED WATER, touch the edges of slide on a paper towel to remove excess water. Place slides on a flat surface away from light. 3. Turn the vial upside down and open the dropper to remove any air bubbles. 4. Apply 2-3 drops of mounting medium directly on top of the specimen. 5. Let stand at room temperature for about 3-5 minutes in the dark. 6. Apply cover slip, carefully avoiding air bubbles. 7. The specimen is ready for visualization under a microscope. 8. One can seal the edges of the cover slip with nail polish, any organic mounting medium or our Organo mounting medium (cat. # AR-6504). If a coverslip is not sealed, air bubbles will appear in few days. 9. Method for applying Coverslip: Put 1-2 drops of FLS on the specimen. After 3-4 minutes apply coverslip carefully avoiding air bubbles. Put Kimwipes on the top of coverslip, press gently to remove excess mounting medium. With 200 micropipette add organic mounting medium to seal the edges. Incubate at 37C for one hour in the dark to dry organic mounting medium. 10. For long term storage it is recommended that the slide be stored in the dark at 2-8oC.

Fluoroshield with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine, Redox. Phycoerythrin (RP-E), Phycocyanin (PC), and Allophycocyanin (APC). Fluorescence is retained during prolonged storage at 4oC in the dark. This medium does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes, RP-E, PC and APC. This mounting medium is fortified with DAPI which is a counter-stain for DNA. This product is to be used in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360nm and emits at 460nm, producing a blue fluorescence. RNA is also stained with DAPI. May encounter problems with FROZEN BRAIN OR OTHER FROZEN TISSUES WITH LOTS OF FAT