Chlorpromazine ELISA Kit

Chlorpromazine is a phenothiazine that belongs to the class of antipsychotic drugs. It acts as a dopamine antagonist and possesses anti-serotonin and anti-histaminergic activity. It is generally used to treat psychotic disorders such as schizophrenia, bipolar disorder, and amphetamine-induced psychosis. It is also used to treat attention deficit hyperactivity disorder in children. In food-producing animals, this drug is used to calm animals. It is also used as feed additives to promote the growth of animals. However, daily use could lead to the accumulation of the drug in animal bodies which may adversely affect the circulatory and nervous system of animals. BioVision’s Chlorpromazine ELISA kit is used to quantitatively measure Chlorpromazine in tissue samples. The kit is based on the Competitive ELISA principle. Samples and standards are added to the microwell plate that is pre-coated with an antigen and competes for binding to the anti-Chlorpromazine antibody. The HRP conjugate is added to each well and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of substrate reagents. Finally, the reaction is terminated with an acidic stop solution. The color developed is inversely proportional to the concentration of Chlorpromazine in the samples.