D-Luciferin
D-luciferin is the natural substrate of the enzyme luciferase (Luc) that catalyzes the production of the typical yellowgreen light of fireflies. The 560 nm chemiluminescence from this reaction peaks within seconds, with light output that is proportional to luciferase concentration when the substrate luciferin is present in excess. The luciferase (luc) gene is a popular reporter gene for research and agent screening. Chemiluminescent techniques are virtually background-free, making the luc reporter gene ideal for detecting low-level gene expression. As little as 0.02 pg of luciferase can be reliably measured in a standard scintillation counter. In addition to its role as a reporter of gene expression, luciferase is commonly used in an extremely sensitive assay for ATP[1]. We of er the firefly luciferase (HY-P1004A), luciferin free acid (HY-12591A), as well as its water-soluble sodium salts (HY-12591) and potassium salts (HY-12591B) .
Product Specifications
CAS Number
[2591-17-5]
Product Name Alternative
D- (-) -Luciferin; Firefly luciferin; Beetle Luciferin
UNSPSC
12171500
Hazard Statement
H302, H315, H319, H335
Target
Fluorescent Dye
Type
Dye Reagents
Related Pathways
Others
Field of Research
Others
Assay Protocol
https://www.medchemexpress.com/D-Luciferin.html
Purity
99.87
Solubility
DMSO : 125 mg/mL (ultrasonic) |H2O : 9.09 mg/mL (ultrasonic; adjust pH to 9 with 1M NaOH)
Smiles
O=C([C@@H]1N=C(C2=NC3=CC=C(O)C=C3S2)SC1)O
Molecular Formula
C11H8N2O3S2
Molecular Weight
280.33
Precautions
H302, H315, H319, H335
References & Citations
Shipping Conditions
Room Temperature
Storage Conditions
4°C (Powder, protect from light)
Scientific Category
Dye Reagents
Clinical Information
No Development Reported
Citation 01
Available Sizes
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