X-alpha-Gal

X-alpha-Gal is a chromogenic substrate that is used to screen colonies with high activity of β-galactopyranoside. X-alpha-Gal also detects α-galactosidase activity. X-alpha-Gal can be used in yeast two hybrid screening for MEL1 gene activation. X-alpha-Gal can be used with Matchmaker GAL4-based products to confirm protein interactions[1][2][3].

Product Specifications

CAS Number

[107021-38-5]

Product Name Alternative

NSC144303

UNSPSC

12352005

Hazard Statement

H302, H315, H319, H335

Target

Biochemical Assay Reagents

Type

Reference compound

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/x-alpha-gal.html

Purity

99.94

Solubility

DMSO : 100 mg/mL (ultrasonic)

Smiles

O[C@@H]1[C@@H](O)[C@@H](O)[C@H](O[C@@H]1OC2=CNC3=C2C(Cl)=C(C=C3)Br)CO

Molecular Formula

C14H15BrClNO6

Molecular Weight

408.63

Precautions

H302, H315, H319, H335

References & Citations

[1]ALAzzE A Y, et al. The effect of chemical mutagenesis on the activity of a-and ß-galactosidases in Lactobacillus reuteri [J]. Milchwissenschaft, 2011, 66: 3.|[2]Rac, A., & Fulgosi, H. (2019) . Qualitative and quantitative dataset of TROL protein interaction with C3 and C4 ferredoxin: NADP+ oxidoreductases. Data in brief, 28, 105038.|[3]Aho, S., et al., (1997) . A novel reporter gene MEL1 for the yeast two-hybrid system. Analytical biochemistry, 253 (2), 270–272.