VEGFR-2/KDR

VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs) . All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. Human VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis.

Product Specifications

Synonyms

Vascular endothelial growth factor receptor-2; KDR; FLK1; CD309; VEGF receptor 2; VEGFR2; kinase insert domain protein receptor

NCBI Gene ID

3791

UniProt

P35968

Accession Number

NP_002244.1

Accession Number mRNA

NM_002253.2

Chromosomal Location

4q11-q12

Reactivity

Anti-Human

Cross Reactivity

Human

Target Antigen

Human VEGFR-2 N-terminal fragment

Clone

(#2F46)

Applications

WB, IHC, ELISA

Purification Method

Protein G chromatography

Assay Protocol

Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

Bioactivity

WB: 1:400-1000; IHC: 1:100-400; ELISA: 1:50

Form

Lyophilized

Buffer

PBS

Reconstitution

PBS

Storage Conditions

Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.