VEGFR-2/Flk-1

The antibody recognizes mouse vascular endothelial growth factor receptor 2, alos known as CD309, VEGFR2, KDR, protein tyrosine kinase receptor flk-1, and fetal liver kinase-1. Flk-1 is a member of the tyrosine protein kinase family, sub-family CSF-1/PDGF, that contains a single pass transmembrane receptor with a protein kinase domain and seven immunoglobulin-like domains in the extracellular region. Flk-1 is expressed at high levels in adult heart, lung, kidney, brain, and skeletal muscle; other tissues express at lower levels. Flk-1 is a receptor for VEGF-A or fully processed VEGF-C; ligand binding plays a key role in vascular development and vascular permeability.

Product Specifications

Synonyms

Soluble vascular endothelial growth factor receptor-2 ; sKdr; sLy73; sFlk-1; sVEGFR2; sVEGF receptor 2; skinase insert domain protein receptor

NCBI Gene ID

16542

UniProt

P35918

Accession Number

ACJ66293.1

Accession Number mRNA

EU884114

Chromosomal Location

5 C3.3; 5 42.0 cM

Reactivity

Anti-Mouse

Cross Reactivity

Mouse

Target Antigen

Recombinant mouse esFlk-1 (RT Cat# S01-M04)

Clone

Rabbit IgG

Applications

Purification Method

Protein A purified

Assay Protocol

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

Bioactivity

Western Blot: use at 1-5 µg/ml.

Form

Lyophilized

Buffer

PBS

Reconstitution

Water

Storage Conditions

The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.