VCAM-1

The antibody reacts with mouse CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and upregulate it upon cytokine stimulation. CD106 binds to integrin alpha4beta1 (VLA-4, CD49d/CD29) and Integrin a4b7 (LPAM-1) and these interactions in the bone marrow and thymus are important for early lymphocyte and myeloid development. Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role for this antigen in the inflammatory response.

Product Specifications

Synonyms

CD106; Vascular Cell Adhesion Protein 1; V-CAM 1

NCBI Gene ID

22329

UniProt

P29533

Accession Number

NP_035823.3

Accession Number mRNA

NM_011693.3

Chromosomal Location

3 G1; 3 50.8 cM

Reactivity

Anti-Mouse

Cross Reactivity

Mouse

Target Antigen

Cytokine stimulated mouse endothelial cells

Clone

(#MAB0804)

Applications

WB, IHC (P), IP

Purification Method

Protein A/G chromatography

Assay Protocol

Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

Bioactivity

WB: 1:100-1000; IHC (P) : 1:25-100

Form

Lyophilized

Buffer

PBS

Reconstitution

PBS (sterile)

Storage Conditions

Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.