TIE-1

TIE-1 (tyrosine kinase with Ig and EGF homology domains 1) and TIE-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF) -like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.

Product Specifications

Synonyms

TIE1; TIE; JTK14

NCBI Gene ID

7075

UniProt

P35590

Accession Number

NP_005415

Accession Number mRNA

NM_005424

Chromosomal Location

1p34-p33

Reactivity

Anti-Human

Cross Reactivity

Human

Target Antigen

Recombinant human soluble TIE-1

Clone

(#8C9)

Applications

ELISA, WB

Purification Method

Protein G chromatography

Assay Protocol

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

Bioactivity

ELISA: Use at 1-15 µg/ml; Western blotting: Use at 1-2 µg/ml

Form

Lyophilized

Buffer

PBS

Reconstitution

Water

Storage Conditions

The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

Notes

The monoclonal antibody will detect native human TIE-1 in ELISA experiments and on the surface of different human cell types. The antibody can be used for ELISA experiments, Western blotting, FACS and cell sorting.