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Prox-1

Prox-1 is a homeobox gene and acts as a master switch for lymphatic endothelial phenotype. Expression of Prox-1 in blood endothelial cells induces expression of other lymphatic marker genes. Together with Podoplanin, Prox-1 can be used to reliably distinguish lymphatic vessels from blood vessels. Prox-1 is expressed in CNS, eye, pancreas, liver and heart, and it is one of the most specific and reliable markers for lymphatic endothelial cells. Produced from sera of rabbits immunized with the recombinant highly conserved C-terminal part of the homeobox transcription factor Prox-1.

Product Specifications

Synonyms

PROX1; W117m

NCBI Gene ID

5629

UniProt

Q92786

Accession Number

NP_002754.2

Accession Number mRNA

NM_002763.3

Chromosomal Location

1q32.2-q32.3

Reactivity

Anti-Human

Cross Reactivity

Human

Target Antigen

Recombinant human Prox-1 (RT #300-052)

Clone

Rabbit IgG

Applications

WB, IF

Purification Method

Antigen-affinity purified

Assay Protocol

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

Bioactivity

Western Blot: Use 1-5 µg/ml

Form

Lyophilized

Buffer

0.5X PBS, pH 7.2

Reconstitution

Water

Storage Conditions

The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

Host or Source

Rabbit

Frequently Asked Questions

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