Prox-1 (fragment)

Prox-1 is a homeobox gene and acts as a master switch for lymphatic endothelial phenotype. Expression of Prox-1 in blood endothelial cells induces expression of other lymphatic marker genes. Together with Podoplanin, Prox-1 can be used to reliably distinguish lympathic vessels from blood vessels. Prox1 is expressed in CNS, eye, pancreas, liver and heart, and it is one of the most specific and reliable markers for lymphatic endothelial cells. The highly conserved C-terminal part of the homeobox transcription factor Prox1 was produced in E. coli. It was not tested for activity and can be used as positive control e.g. in Western analysis.

Product Specifications

Synonyms

PROX1; W117m

NCBI Gene ID

5629

UniProt

Q92786

Accession Number

NP_002754

Accession Number mRNA

NM_002763

Chromosomal Location

1q32.2-q32.3

Reactivity

Human

Cross Reactivity

Human

Sequence

MAEGLSLSLIKSECGDLQDMSEISPYSGSAMQEGLSPNHLKKAKLMFFYTRYPSSNMLKTYFSDVKFNRCITSQLIKWFSNFREFYYIQMEKYARQAINDGVTSTEELSITRDCELYRALNMHYNKANDFEVPERFLEVAQITLREFFNAIIAGKDVDPSWKKAIYKVICKLDSEVPEIFKSPNCLQELLHE

Assay Protocol

Centrifuge vial prior to opening. The lyophilized Prox-1 should be reconstituted in water to a concentration not lower than 50 µg/ml.

Purity

> 95% by SDS-PAGE

Bioactivity

Control for Western Blotting. Biological activity not tested.

Length

192

Form

Lyophilized

Buffer

PBS

Reconstitution

Water

Molecular Weight

22.35 kDa

Storage Conditions

The lyophilized protein is stable for a few weeks at room temperature, but best stored at –20°C. Reconstituted Prox-1 should be stored in working aliquots at –20°C. Avoid repeated freeze-thaw cycles.