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Prolactin

Recombinant rabbit prolactin, one polypeptide chain containing 199 amino and an additional Ala at N-terminus acids and having a molecular mass of ~ 23 kDa, was purified by proprietary chromatographic techniques (unpublished data) .

Product Specifications

Synonyms

Mammotropin, Luterotropic hormone, Lutetropin

NCBI Gene ID

100009394

UniProt

Q28632

Accession Number

NP_001076144.1

Accession Number mRNA

NM_001082675.1

Reactivity

Rabbit

Cross Reactivity

Rabbit

Sequence

APICPSGAVNCQVSLRDLFDRAVILSHHIHKLSSEMFNEFDKRYTQGRGFITKAINSCHTSSLSTPEDKEQAQQIHHEDLLNMVLRVLPSWNDPLYHLVTEVRGMQEAPDAILSKAIEIEEQNRRLLEGMEKIVGQVHPGIKENEIYSVWSGLPSLQMADEDARLFAFYNLLHCLRRDSHKIDNYLKLLKCRIIYDSNC

Assay Protocol

It is recommended to reconstitute the lyophilized rbPRL in sterile 0.04% NaHCO3 adjusted, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Endotoxin

< 0.1 ng/µg of protein (< 1EU/µg)

Purity

> 98% by SDS-PAGE & HPLC analyses

Bioactivity

Recombinynt rbPRL is fully biologically active as evidenced by inducing proliferation of Nb2 cells and Baf3 cells stably transfected with rabbit prolactin receptor, algough its relative activity as compared to human prolactin is respectively 8-fold and 4-fold lower. Rabbit PRL forms also 1:1 complex with rabbit PRLR-ECD.

Length

199

Form

Lyophilized

Reconstitution

0.4% NaHCO3, pH 8-9

Molecular Weight

23.0 kDa

Host or Source

E. coli

N Terminal Sequence

APICP

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