Prolactin antagonist (G129R)
Recombinant ovine prolactin, G129R mutein is one polypeptide chain containing 199 amino and an additional Ala at N-terminus acids and having a molecular mass of ~ 23 kDa, was purified by proprietary chromatographic techniques (no published information) . It is available in two forms: as full size protein and it its DEL 9 aa truncated form from its N-terminus which has higher inhibitory activity.
Product Specifications
Synonyms
Mammotropin, Luterotropic hormone, Lutetropin
NCBI Gene ID
443317
UniProt
P01240
Accession Number
NP_001009306.1
Accession Number mRNA
NM_001009306.1
Reactivity
Ovine
Cross Reactivity
Ovine
Sequence
ATPVCPNGPGNCQVSLRDLFDRAVMVSHYIHNLSSEMFNEFDKRYAQGKGFITMALNSCHTSSLPTPEDKEQAQQTHHEVLMSLILGLLRSWNDPLYHLVTEVRGMKGVPDAILSRAIEIEEENKRLLERMEMIFGQVIPGAKETEPYPVWSGLPSLQTKDEDARHSAFYNLLHCLRRDSSKIDTYLKLLNCRIIYNNNC
Assay Protocol
It is recommended to reconstitute the lyophilized oPRL G129R in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
Endotoxin
< 0.1 ng/µg of protein (< 1EU/µg)
Purity
> 99% by SDS-PAGE & HPLC analyses
Bioactivity
Recombinant oPRL G129R is devoid of agonistic activity and capable of inhibiting biological activity of oPRL or other lactogenic hormones as evidenced by proliferation assay of Nb2 or other cells. The truncated form is more potent inhibitor.
Length
199
Form
Lyophilized
Reconstitution
Water
Molecular Weight
23.0 kDa
Host or Source
E. coli
N Terminal Sequence
ATPVCP
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