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Prolactin Recombinant Protein

Recombinant chickenn prolactin, (chPRL) one polypeptide chain containing 199 amino and an additional Ala at N-terminus acids and having a molecular mass of ~ 23 kDa, was purified by proprietary chromatographic techniques as described by Oclon et al (2017) GCE 240, 27-34.

Product Specifications

CAS Number

9000-83-3

Synonyms

Mammotropin, Luterotropic hormone, Lutetropin

NCBI Gene ID

396453

UniProt

P14676

Accession Number

NP_990797.2

Accession Number mRNA

NM_205466.3

Host

E. coli

Origin Species

Chicken

Species Reactivity

Chicken

Sequence

ALPICPIGSVNCQVSLGELFDRAVKLSHYIHYLSSEIFNEFDERYAQGRGFITKAVNGCHTSSLTTPEDKEQAQQIHHEDLLNLVVGVLRSWNDPLIHLASEVQRIKEAPDTILWKAVEIEEQNKRLLEGMEKIVGRVHSGDAGNEIYSHWDGLPSLQLADEDSRLFAFYNLLHCLRRDSHKIDNYLKVLKCRLIHDSNC

Detection Range

Recombinant hPRL is biologically active as evidenced by inducing proliferation of Nb2 cells and baf/3 cells stably transfected with hPRL receptors and by in vivo in chicken.

Endotoxin

< 0.1 ng/µg of protein (<1EU/µg)

Purity

> 95% by SDS-PAGE & HPLC analyses

Length

199

Form

Lyophilized

Reconstitution

It is recommended to reconstitute the lyophilized chPRL in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.

Molecular Weight

23.0 kDa

Shipping Conditions

Room Temperature

N Terminal Sequence

ALPICP

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