Prolactin Recombinant Protein
Recombinant chickenn prolactin, (chPRL) one polypeptide chain containing 199 amino and an additional Ala at N-terminus acids and having a molecular mass of ~ 23 kDa, was purified by proprietary chromatographic techniques as described by Oclon et al (2017) GCE 240, 27-34.
Product Specifications
CAS Number
9000-83-3
Synonyms
Mammotropin, Luterotropic hormone, Lutetropin
NCBI Gene ID
396453
UniProt
P14676
Accession Number
NP_990797.2
Accession Number mRNA
NM_205466.3
Host
E. coli
Origin Species
Chicken
Species Reactivity
Chicken
Sequence
ALPICPIGSVNCQVSLGELFDRAVKLSHYIHYLSSEIFNEFDERYAQGRGFITKAVNGCHTSSLTTPEDKEQAQQIHHEDLLNLVVGVLRSWNDPLIHLASEVQRIKEAPDTILWKAVEIEEQNKRLLEGMEKIVGRVHSGDAGNEIYSHWDGLPSLQLADEDSRLFAFYNLLHCLRRDSHKIDNYLKVLKCRLIHDSNC
Detection Range
Recombinant hPRL is biologically active as evidenced by inducing proliferation of Nb2 cells and baf/3 cells stably transfected with hPRL receptors and by in vivo in chicken.
Endotoxin
< 0.1 ng/µg of protein (<1EU/µg)
Purity
> 95% by SDS-PAGE & HPLC analyses
Length
199
Form
Lyophilized
Reconstitution
It is recommended to reconstitute the lyophilized chPRL in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
Molecular Weight
23.0 kDa
Shipping Conditions
Room Temperature
N Terminal Sequence
ALPICP
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