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Prolactin, antagonist (del 1-9) G129R

Prolactin, also known as mammotrophin, luterotropic hormone, and lutetropin, is a neuroendocrine hormone secreted by the pituitary gland. Its primary function is to promote and maintain lactation during pregnancy and suckling. In addition, Prolactin plays an immune-regulatory role by stimulating the activities of ornithine decarboxylase and protein kinase C, which are important for the proliferation, differentiation, and function of lymphocytes. Recombinant human prolactin antagonist del 1-9 G129R mutant has a molecular weight of approx. 22 kDa and contains 190 amino acid residues with an additional N-terminal Alanin.

Product Specifications

Synonyms

Mammotropin, Luterotropic hormone, Lutetropin, PRL

NCBI Gene ID

5617

UniProt

P01236

Accession Number

NP_000939.1

Accession Number mRNA

NM_000948

Chromosomal Location

6p22.2-p21.3

Reactivity

Human

Cross Reactivity

Human

Sequence

MLPICPGGAA RCQVTLRDLF DRAVVLSHYI HNLSSEMFSE FDKRYTHGRG FITKAINSCH TSSLATPEDK EQAQQMNQKD FLSLIVSILR SWNEPLYHLV TEVRGMQEAP EAILSKAVEI EEQTKRLLEG MELIVSQVHP ETKENEIYPV WSGLPSLQMA DEESRLSAYY NLLHCLRRDS HKIDNYLKLL KCRIIHNNNC

Assay Protocol

It is recommended to reconstitute the lyophilized hPRL in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.

Endotoxin

< 0.1 ng/µg of protein (< 1EU/µg)

Purity

> 97% by SDS-PAGE & Gel Filtration

Bioactivity

Determined by its ability to inhibit hPRL-induced proliferation of Nb2 cells or Baf3 cells stably transfected with hPRL receptors. It also interacts with the hPRL receptor extracellular domain at a 1:1 molar ratio as determined by SEC and SPR analysis.

Length

191

Form

Lyophilized

Reconstitution

NaHCO3

Molecular Weight

22 kDa

Host or Source

E. coli

N Terminal Sequence

ARSQVT

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