NGF-beta

Β-NGF is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. β-NGF is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. β-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. The functional form of human β-NGF is a noncovalently disulfide-linked homodimer, of two 13.5 kDa polypeptide monomers (240 total amino acid residues) . The three disulfide bonds are required for biological activity.

Product Specifications

Synonyms

NGF; NGFB; HSAN5; Beta-NGF

NCBI Gene ID

4803

UniProt

P01138

Accession Number

NP_002497.2

Accession Number mRNA

NM_002506

Chromosomal Location

1p13.1

Reactivity

Human

Cross Reactivity

Chicken, Hamster, Mouse, Rat, Human, Leech

Sequence

SSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRRA

Endotoxin

< 0.1 ng/µg of protein (< 1EU/µg)

Purity

> 98% by SDS-PAGE & HPLC analysis

Bioactivity

Determined by its ability to stimulate chick E9 DRG neurite outgrowth. The ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.