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MIP-4 (CCL18)

MIP-4 is a CC chemokine that is expressed in lymph nodes, lungs, placenta and bone marrow. MIP-4’s primary receptor is unknown. MIP-4 chemoattracts lymphocytes, and has been shown to exert activity on both CD4+and CD8+ T cells. Recombinant human MIP-4 is a 7.8 kDa protein containing 68 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

Product Specifications

Synonyms

Macrophage Inflammatory Protein-4, CCL18, PARC, DC-CK1, AMAC-1

NCBI Gene ID

6362

UniProt

P55774

Accession Number

NP_002979

Accession Number mRNA

NM_002988

Chromosomal Location

17q11.2

Reactivity

Human

Cross Reactivity

Human, Mouse, Bacteria

Sequence

QVGTNKELC CLVYTSWQIP QKFLVDYSET SPQCPKPGVI LLTKRGRQIC ADPNKKWVQK YISDLKLNA

Endotoxin

< 0.1 ng/µg of protein (< 1EU/µg)

Purity

> 98% by SDS-PAGE & HPLC analyses

Bioactivity

Assay #1: Determined by its ability to chemoattract human T lymphocytes using a concentration of 1.0 - 10.0 ng/ml. Assay #2: Determined by its ability to chemoattract human iDCs using a concentration of 1.0 - 10.0 ng/ml.

Length

68

Form

Lyophilized

Reconstitution

Water

Molecular Weight

7.8 kDa

Storage Conditions

For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1 % HSA or 0.1% BSA) and store in working aliquots at -20°C to -80°C. Lyophilized MIP-4 although stable at room temperature for 3 weeks, should be stored desiccated below -20°C. Upon reconstitution MIP-4 can be stored at 4°C between 2-7 days and for future use below -20°C.

Host or Source

E. coli

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