Lyve-1

LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.

Product Specifications

Synonyms

Lyve1; Xlkd1; Lyve-1; Crsbp-1; 1200012G08Rik

NCBI Gene ID

114332

UniProt

Q8BHC0

Accession Number

NP_444477

Accession Number mRNA

NM_053247

Chromosomal Location

7; 7F2

Reactivity

Anti-Mouse

Cross Reactivity

Mouse

Target Antigen

Recombinant mouse soluble Lyve-1 (RT#S01-026)

Clone

Rabbit IgG

Applications

WB, IHC (C), IF, FC

Purification Method

Protein-A purified

Assay Protocol

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

Bioactivity

Western Blot: use at 2-5 µg/ml; FACS: use at 3-10 µg/ml; IF/IHC: applicable with cryo-sections.

Form

Lyophilized

Buffer

PBS

Reconstitution

Water

Storage Conditions

The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.