Lyve-1

LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.

Product Specifications

Synonyms

LYVE1; HAR; XLKD1; LYVE-1; CRSBP-1

NCBI Gene ID

10894

UniProt

Q9Y5Y7

Accession Number

NP_006682

Accession Number mRNA

NM_006691

Chromosomal Location

11p15

Reactivity

Anti-Human

Cross Reactivity

Human

Target Antigen

Recombinant human LYVE-1 (RT #S01-028)

Clone

Rabbit IgG

Applications

WB, IHC (P, F), IF

Purification Method

Protein-A purified

Assay Protocol

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

Bioactivity

Western Blot: Use 1-5 µg/ml

Form

Lyophilized

Buffer

PBS

Reconstitution

Water

Storage Conditions

The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.