What is the catalog number for Leptin B Recombinant Protein?

The catalog number for Leptin B Recombinant Protein is 500-075S.

Is Leptin B Recombinant Protein in stock?

Leptin B Recombinant Protein is currently made to order / not held in immediate stock. Contact us for lead time.

Leptin B Recombinant Protein

Full‐length cDNA encoding two leptin sequences (tLepA and tLepB) were identified in tilapia (Oreochromis niloticus). The cDNAs of tLepA and tLepB were 486 bp and 459 bp in length, encoding proteins of 161 aa and 152 aa, respectively. The tLepB ‐expressing plasmid was transformed into E. coli and expressed as recombinant proteins upon induction with nalidixic acid, found almost entirely in insoluble inclusion bodies (IBs). The protein was solubilized, refolded and purified to homogeneity by anion‐exchange chromatography. More information can be found in Shpilman et al. (2014) General and Comparative Endocrinology 270, 74-85.

Product Specifications

CAS Number

9000-83-3

Synonyms

Lep; ob; obese

NCBI Gene ID

100704338

UniProt

A0A067Z7V9

Accession Number

NP_001287978.1

Accession Number mRNA

NM_001301049.1

Host

E. coli

Origin Species

Tilapia

Species Reactivity

Tilapia

Sequence

ALLTKGESIKNTIHNIVNIAQITLVHIKKLKLPATPTEVPTPSIDGLSSISHDLGVLDNELQHPFLIQIQADVSSLEGRVRSFALSMECPLKPKPAVQTDESVFPDSRLYMTVAKVQHYLEKLILNKGKLKLC

Detection Range

Monomeric and dimeric Tilapia leptins were biologically active in promoting proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor (hLepR), but their activity was four orders of magnitude lower than that of mammalian leptin. Furthermore, the Tilapia leptins were biologically active in promoting STAT‐LUC activation in COS7 cells transfected with Tilapia leptin receptor but not in cells transfected with human leptin receptor. Tilapia Leptin A was more active than Tilapia Leptin B.

Endotoxin

< 0.1 ng/µg of protein (<1EU/µg)

Purity

> 95.0% as determined by Gel filtration and SDS-PAGE gel.

Length

133

Form

Lyophilized

Reconstitution

It is recommended to reconstitute the lyophilized recombinant Tilapia leptin B in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted with other aqueous solutions.