Leptin Recombinant Protein
Recombinant salamander leptin (Sal-LEP)is a single polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kD. Recombinant salamander leptin was purified by proprietary chromatographic techniques, similarly to that described previously by Salomon et al (2006) Protein Expression and Purifcation 47, 128–136.
Product Specifications
CAS Number
9000-83-3
Synonyms
Lep; ob; obese
Host
E. coli
Origin Species
Salamander
Species Reactivity
Salamander
Detection Range
Recombinant salamander leptin is fully biologically active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. The activity of recombinant salamander leptin in that test is similar to that of mouse leptin (see Salomon et al (2006) Protein Expression and Purifcation 47, 128–136).
Endotoxin
< 0.1 ng/µg of protein (<1EU/µg)
Purity
> 99.0% as determined by Gel filtration and SDS-PAGE gel.
Length
146
Form
Lyophilized
Reconstitution
It is recommended to reconstitute the lyophilized recombinant salamander leptin in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml. Recombinant salamander leptin , which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
Molecular Weight
16.0 kDa
Shipping Conditions
Room Temperature
N Terminal Sequence
AIMVDQLRMD
Frequently Asked Questions
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