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Leptin

Recombinant rabbit leptin, is one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa. Recombinant rabbit leptin was purified by proprietary chromatographic techniques, (Solomon, Charlier, Djiane and Gertler, . unpublished results)

Product Specifications

Synonyms

Lep; ob; obese

NCBI Gene ID

100008747

UniProt

B0KZL0

Accession Number

NP_001156541.1

Accession Number mRNA

NM_001163069.1

Reactivity

Rabbit

Cross Reactivity

Rabbit

Sequence

AVPMRKVQDDTKTLIKTIVTRISDISHTQSVSSRQRVVGLDFIPGLHPNLSLSTMDQTLAIYQQILASLPSRNVIQIANDLENLRDLLHLLASSKSCPLPRASGLETLEGLGGVLEASLYSTEVVALSRLQGFLQAMLQQLDLGPGC

Assay Protocol

It is recommended to reconstitute the lyophilized recombinant rabbit leptin in sterile 0.4% NaHCO3 adjusted, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Endotoxin

< 0.1 ng/µg of protein (< 1EU/µg)

Purity

> 97.0% as determined by Gel filtration and SDS-PAGE gel.

Bioactivity

Recombinant rabbit leptin is fully biologically active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.

Length

146

Form

Lyophilized

Reconstitution

0.4% NaHCO3, pH 8-9

Molecular Weight

16.0 kDa

Host or Source

E. coli

N Terminal Sequence

AVPI

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