Leptin
Recombinant rabbit leptin, is one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa. Recombinant rabbit leptin was purified by proprietary chromatographic techniques, (Solomon, Charlier, Djiane and Gertler, . unpublished results)
Product Specifications
Synonyms
Lep; ob; obese
NCBI Gene ID
100008747
UniProt
B0KZL0
Accession Number
NP_001156541.1
Accession Number mRNA
NM_001163069.1
Reactivity
Rabbit
Cross Reactivity
Rabbit
Sequence
AVPMRKVQDDTKTLIKTIVTRISDISHTQSVSSRQRVVGLDFIPGLHPNLSLSTMDQTLAIYQQILASLPSRNVIQIANDLENLRDLLHLLASSKSCPLPRASGLETLEGLGGVLEASLYSTEVVALSRLQGFLQAMLQQLDLGPGC
Assay Protocol
It is recommended to reconstitute the lyophilized recombinant rabbit leptin in sterile 0.4% NaHCO3 adjusted, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Endotoxin
< 0.1 ng/µg of protein (< 1EU/µg)
Purity
> 97.0% as determined by Gel filtration and SDS-PAGE gel.
Bioactivity
Recombinant rabbit leptin is fully biologically active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.
Length
146
Form
Lyophilized
Reconstitution
0.4% NaHCO3, pH 8-9
Molecular Weight
16.0 kDa
Host or Source
E. coli
N Terminal Sequence
AVPI
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