Leptin Recombinant Protein
Selected Variant
500-071S
Size: 100 µg
The first biologically active recombinant fish leptin was prepared according to the sequence published by Kurokawa et al. (2005)Peptides 26, 745-750 in two forms: monomer and covalent dimer. MS analysis revealed molecular masses of 15,291 and 30,585 Da, close to the theoretical values of 15,270 and 30,540 Da. CD spectra revealed high similarity to mammalian leptins. Other details of its preparation will be soon published by Yacobovitz et al , General and Comparative Endocrinology (2008) 156(1):83-90.
Product Specifications
CAS Number
9000-83-3
Synonyms
Lep; ob; obese
NCBI Gene ID
548631
UniProt
Q588G0
Accession Number
NP_001027897.1
Accession Number mRNA
NM_001032725.1
Host
E. coli
Origin Species
Pufferfish
Species Reactivity
Pufferfish
Sequence
ALPGALDAMDVEKMKSKVTWKAQGLVARIDKHFPDRGLRFDTDKVEGSTSVVASLESYNNLISDRFGGVSQIKTEISSLAGYLNHWREGNCQEQQPKVWPRRNIFNHTVSLEALMRVREFLKLLQKNVDLLERC
Detection Range
Endotoxin
< 0.1 ng/µg of protein (<1EU/µg)
Purity
> 98.0% as determined by Gel filtration and SDS-PAGE gel.
Length
134
Form
Lyophilized
Reconstitution
Molecular Weight
15.3 kDa (momnomer)
Shipping Conditions
Room Temperature
N Terminal Sequence
ALPGA
Price
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