What is the catalog number for Leptin R4C mutant Recombinant Protein?

The catalog number for Leptin R4C mutant Recombinant Protein is 500-064S.

Is Leptin R4C mutant Recombinant Protein in stock?

Leptin R4C mutant Recombinant Protein is currently made to order / not held in immediate stock. Contact us for lead time.

Leptin R4C mutant Recombinant Protein

Recombinant ovine leptin, one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa. The wild type leptin was mutated at position 4 replacing arginine by cysteine. Recombinant ovine leptin R4C mutant was purified by proprietary chromatographic techniques, see Reicher et al. J. Animal Sciences 90:410-418 (2012).

Product Specifications

CAS Number

9000-83-3

Synonyms

Lep; ob; obese

NCBI Gene ID

443534

Accession Number

XP_027824581.2

Accession Number mRNA

XM_027968780.2

Host

E. coli

Origin Species

Ovine

Species Reactivity

Ovine

Sequence

AVPCRKVQDDTKTLIKTIVTRINDISHTQSVSSKQRVTGLDFIPGLHPLLSLSKMDQTLAIYQQILASLPSRNVIQISNDLENLRDLLHLLAASKSCPLPQVRALESLESLGVVLEASLYSTEVVALSRLQGSLQDMLRQLDLSPG

Detection Range

Recombinant ovine leptin R4C mutant is fully biologically active compared to the wild-type ovine leptin as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.

Endotoxin

< 0.1 ng/µg of protein (<1EU/µg)

Purity

> 98.0% as determined by Gel filtration and SDS-PAGE gel.

Length

146

Form

Lyophilized

Reconstitution

It is recommended to reconstitute the lyophilized recombinant ovine leptin R4C mutant in sterile 0.4% NaHCO3 adjusted, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.