Leptin Recombinant Protein

Recombinant horse leptin is an one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa. Recombinant horse leptin was purified by proprietary chromatographic techniques, (unpublished).

Product Specifications

CAS Number

9000-83-3

Synonyms

Lep; ob; obese

NCBI Gene ID

100034042

UniProt

A0A3Q2L864

Accession Number

NP_001157452.1

Accession Number mRNA

NM_001163980.1

Host

E. coli

Origin Species

Horse

Species Reactivity

Horse

Sequence

AVPIRKVQDDTKTLIKTIVTRINDISHTQSVSSKQRVTGLDFIPGLHPVLSLSKMDQTLAIYQQILTSLPSRNVIQISNDLENLRDLLHLLASSKSCPLPQARGLETLASLGGVLEASLYSTEVVALSRLQGSLQDMLQQLDLSPGC

Detection Range

Recombinant horse leptin is fully biologically active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.

Endotoxin

< 0.1 ng/µg of protein (<1EU/µg)

Purity

> 99.0% as determined by Gel filtration and SDS-PAGE gel.

Length

146

Form

Lyophilized

Reconstitution

It is recommended to reconstitute the lyophilized recombinant horse leptin in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.