Leptin Recombinant Protein
Selected Variant
500-060
Size: 500 µg
Recombinant dog leptin is an one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa. Dog leptin was purified by proprietary chromatographic techniques, (Solomon, Charlier, Djiane and Gertler, unpublished results).
Product Specifications
CAS Number
9000-83-3
Synonyms
Lep; ob; obese
NCBI Gene ID
403616
UniProt
O02720
Accession Number
NP_001003070.1
Accession Number mRNA
NM_001003070.1
Host
E. coli
Origin Species
Dog
Species Reactivity
Dog
Sequence
AVPIRKVQDDTKTLIKTIVARINDISHTQSVSSKQRVAGLDFIPGLQPVLSLSRMDQTLAIYQQILNSLHSRNVVQISNDLENLRDLLHLLASSKSCPLPRARGLETFESLGGVLEASLYSTEVVALNRLQAALQDMLRRLDLSPGC
Detection Range
Recombinant dog leptin is fully biologically active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.
Endotoxin
< 0.1 ng/µg of protein (<1EU/µg)
Purity
> 98.0% as determined by Gel filtration and SDS-PAGE gel.
Length
146
Form
Lyophilized
Reconstitution
It is recommended to reconstitute the lyophilized recombinant dog leptin in sterile 0.4% NaHCO3 adjusted, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Molecular Weight
16.0 kDa
Shipping Conditions
Room Temperature
N Terminal Sequence
AVPIR
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