Leptin binding protein Recombinant Protein

Recombinant chicken leptin binding domain (chLBD), is one polypeptide chain containing 208 amino acids and an additional Ala at N-terminus acids. It consists of the cytokine binding domain of leptin receptor (amino acids 420-626 of chicken leptin receptor and having a molecular mass of ~ 24.5 kDa, It was purified by proprietary chromatographic techniques (see Niv-Spector et al. Biochemical Journal (2005) 390:475-484.

Product Specifications

CAS Number

9000-83-3

Synonyms

Lep; ob; obese

NCBI Gene ID

374223

UniProt

Q9I8V6

Accession Number

NP_989654.2

Accession Number mRNA

NM_204323.2

Host

E. coli

Origin Species

Chicken

Species Reactivity

Chicken

Sequence

AIDVNINIKCETDGYLTKMTCRWSANPNALLLGSSLQLKYHMSKIYCSNFPSTPPESEVKECHFQRNHSYECTFQPVFLLSGYTMWIELKHSLGTLESSPTCVVPADVVKPLPPSNIKAEITRNDGLLNVSWTNPVFTNDDLKFQIRYAVNREELTWELYEVLSVPTRSAVIEVQLCVEYIVQIRCRALDGLGYWSNWSRSAYAAVKD

Detection Range

Recombinant chLBDL is fully biologically active as evidenced by high affinity binding of mammalian leptins at 1:1 molar ratio.

Endotoxin

< 0.1 ng/µg of protein (<1EU/µg)

Purity

> 99.0% as determined by Gel filtration and SDS-PAGE gel.

Length

208

Form

Lyophilized

Reconstitution

It is recommended to reconstitute the lyophilized chLBD in sterile water at pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.