Leptin Recombinant Protein

Recombinant chicken leptin is an one polypeptide chain containing 145 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa. Chicken leptin was prepared according to the sequence published by the groups of Taouis and McMutry, purified by proprietary chromatographic techniques, see Raver et al. Protein Expr Purif. 1998 Dec; 14(3):403-8.

Product Specifications

CAS Number

9000-83-3

Synonyms

Lep; ob; obese

NCBI Gene ID

373955

UniProt

O42164

Accession Number

APC23099.1

Accession Number mRNA

KT970642.1

Host

E. coli

Origin Species

Chicken

Species Reactivity

Chicken

Sequence

AVPCQIFQDDTKTLIKTIVTRINDISHTSVSAKQRVTGLDFIPGLHPILSLSKMDQTLAVYQQVLTSLPSQNVLQIANDLENLRDLLHLLAFSKSCSLPQTSGLQKPESLDGVLEASLYSTEVVALSRLQGSLQDILQQLDISPEC

Detection Range

Recombinant chicken leptin is biologically active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Its activity is however 5-10 fold lower as compared to mammalian leptins.

Endotoxin

< 0.1 ng/µg of protein (<1EU/µg)

Purity

> 98.0% as determined by Gel filtration and SDS-PAGE gel.

Length

146

Form

Lyophilized

Reconstitution

It is recommended to reconstitute the lyophilized recombinant chicken leptin in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.