Leptin
Recombinant bovine leptin is an one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa. Bovine leptin was purified by proprietary chromatographic techniques, see Raver et al. Prot. Express. Purif. 19, 30-40 (2000) .
Product Specifications
Synonyms
Lep; ob; obese
NCBI Gene ID
280836
UniProt
P50595
Accession Number
NP_776353.2
Accession Number mRNA
NM_173928.2
Reactivity
Bovine
Cross Reactivity
Rat
Sequence
AVPIRKVQDDTKTLIKTIVTRINDISHTQSVSSKQRVTGLDFIPGLHPLLSLSKMDQTLAIYQQILTSLPSRNVVQISNDLENLRDLLHLLAASKSCPLPQVRALESLESLGVVLEASLYSTEVVALSRLQGSLQDMLRQLDLSPGC
Assay Protocol
It is recommended to reconstitute the lyophilized recombinant bovine leptin in sterile 0.4% NaHCO3 adjusted, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Endotoxin
< 0.1 ng/µg of protein (< 1EU/µg)
Purity
> 98.0% as determined by Gel filtration and SDS-PAGE gel.
Bioactivity
Recombinant bovine leptin is fully biologically active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.
Length
146
Form
Lyophilized
Reconstitution
0.4% NaHCO3, pH 8-9
Molecular Weight
16.0 kDa
Host or Source
E. coli
N Terminal Sequence
AVPIR
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