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Leptin antagonist (mutant L39A/D40A/F41A)

Recombinant human leptin is one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa. Recombinant human leptin was mutated, resulting in L39A/D40A/F41A mutant that was purified by proprietary chromatographic techniques. Preparation of leptin antagonists was recently published in Biochemical Journal (Niv-Spector et al, Biochem. J 291;221-230 (2005) .

Product Specifications

Synonyms

Lep; ob; obese

NCBI Gene ID

3952

UniProt

P41159

Accession Number

NP_000221.1

Accession Number mRNA

NM_000230

Reactivity

Human

Cross Reactivity

Human

Sequence

AVPIQKVQDDTKTLIKTIVTRINDISHTQSVSSKQKVTGAAAIPGLHPILTLSKMDQTLAVYQQILTSMPSRNVIQISNDLENLRDLLHVLAFSKSCHLPWASGLETLDSLGGVLEASGYSTEVVALSRLQGSLQDMLWQLDLSPGC

Assay Protocol

It is recommended to reconstitute the lyophilized recombinant human leptin antagonist in sterile 0.4% NaHCO3 adjusted, not less than 100µg/ml, which can then be further diluted to other aqueous solutions preferably in presence of carrier protein.

Endotoxin

< 0.1 ng/µg of protein (< 1EU/µg)

Purity

> 98.0% as determined by Gel filtration and SDS-PAGE gel.

Bioactivity

Recombinant human leptin antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. It also inhibits various leptin effects in several in vitro bioassays.

Length

146

Form

Lyophilized

Reconstitution

0.4% NaHCO3, pH 8-9

Molecular Weight

16.0 kDa

Host or Source

E. coli

N Terminal Sequence

AVPIQ

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