Leptin antagonist (mutant L39A/D40A/F41A)
Recombinant human leptin is one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa. Recombinant human leptin was mutated, resulting in L39A/D40A/F41A mutant that was purified by proprietary chromatographic techniques. Preparation of leptin antagonists was recently published in Biochemical Journal (Niv-Spector et al, Biochem. J 291;221-230 (2005) .
Product Specifications
Synonyms
Lep; ob; obese
NCBI Gene ID
3952
UniProt
P41159
Accession Number
NP_000221.1
Accession Number mRNA
NM_000230
Reactivity
Human
Cross Reactivity
Human
Sequence
AVPIQKVQDDTKTLIKTIVTRINDISHTQSVSSKQKVTGAAAIPGLHPILTLSKMDQTLAVYQQILTSMPSRNVIQISNDLENLRDLLHVLAFSKSCHLPWASGLETLDSLGGVLEASGYSTEVVALSRLQGSLQDMLWQLDLSPGC
Assay Protocol
Endotoxin
< 0.1 ng/µg of protein (< 1EU/µg)
Purity
> 98.0% as determined by Gel filtration and SDS-PAGE gel.
Bioactivity
Length
146
Form
Lyophilized
Reconstitution
0.4% NaHCO3, pH 8-9
Molecular Weight
16.0 kDa
Host or Source
E. coli
N Terminal Sequence
AVPIQ
Frequently Asked Questions
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