What is the catalog number for Leptin N82K mutant Recombinant Protein?

The catalog number for Leptin N82K mutant Recombinant Protein is 500-039.

Is Leptin N82K mutant Recombinant Protein in stock?

Leptin N82K mutant Recombinant Protein is currently made to order / not held in immediate stock. Contact us for lead time.

Leptin N82K mutant Recombinant Protein

Recombinant protein - human leptin N82K mutant was produced by site specific mutagenesis. It consists of one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa., Human leptin mutant was purified by proprietary chromatographic techniques, see Niv-Spector et al. (2010) Mol Genet Metab. 100(2):193-7. It is devoid of biological activity and mey serve for control experiments.

Product Specifications

CAS Number

9000-83-3

Synonyms

Lep; ob; obese

NCBI Gene ID

3952

UniProt

P41159

Accession Number

NP_000221.1

Accession Number mRNA

NM_000230

Host

E. coli

Origin Species

Human

Species Reactivity

Human

Sequence

AVPIQKVQDDTKTLIKTIVTRINDISHTQSVSSKQKVTGLDFIPGLHPILTLSKMDQTLAVYQQILTSMPSRNVIQISNDLEKLRDLLHVLAFSKSCHLPWASGLETLDSLGGVLEASGYSTEVVALSRLQGSLQDMLWQLDLSPGC

Detection Range

Recombinant human leptin N82K mutant is less than 0.1% active active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. This abolishment of activity results from drastically reduced affinity toward leptin receptor.

Endotoxin

< 0.1 ng/µg of protein (<1EU/µg)

Purity

> 98.0% as determined by Gel filtration and SDS-PAGE gel.

Length

146

Form

Lyophilized

Reconstitution

It is recommended to reconstitute the lyophilized recombinant human leptin N82K mutant in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.