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Indian Hedgehog N-terminus

Indian Hedgehog (Ihh), Sonic Hedgehog (Shh), and Desert Hedgehog (Dhh) are important signaling molecules during embryonic development and are highly conserved within and across species. Mouse Ihh cDNA encodes a 411 amino acid (aa) polypeptide with a predicted 27 aa signal peptide. Post-translational processing yields a 19 kDa lipid-modified N-terminal domain that is the signaling molecule. At the cell surface, Ihh activity is mediated by binding the 12-pass transmembrane receptor, Patched (Ptc), and signaling through the 7-pass transmembrane G-protein coupled receptor, Smoothened (Smo) .

Product Specifications

Synonyms

IHH; BDA1; HHG2

NCBI Gene ID

16147

UniProt

P97812

Accession Number

NP_034674.1

Accession Number mRNA

NM_010544.2

Reactivity

Anti-Mouse

Cross Reactivity

Mouse

Target Antigen

Mouse recombinant protein of N-Terminal fragment of ihh protein

Clone

(#3A11)

Applications

WB

Purification Method

Protein A/G chromatography

Assay Protocol

Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

Form

Lyophilized

Buffer

PBS

Reconstitution

PBS

Storage Conditions

Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.

Host or Source

Rat

Isotype

IgG2

Frequently Asked Questions

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