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IL-22 pegylated

Mono-pegylated (with 20 kDa PEG) recombinant mouse interleukin 22 is one polypeptide chain containing 147 amino, an additional Ala at N-terminus and one molecule of PEG 20 kDa at its N-terminus, having an expected molecular mass of ~ 36 kDa as determined by MS. However due to enlarged hydrodymanic volume it runs on the SDS-PAGE as 50 kDa protein and in gel-filtration on Superdex 200 as over 200 kDa protein. For more details see L. Niv-Spector et al. Protein Eng Des Sel. (PEDS) 25:397-404 (2012) .

Product Specifications

Synonyms

IL22; IL-22; Iltif; IL-22a; ILTIFa

NCBI Gene ID

50929

UniProt

Q9JJY9

Accession Number

NP_058667.1

Accession Number mRNA

NM_016971

Reactivity

Mouse

Cross Reactivity

Mouse

Label

PEG

Sequence

ALPVNTRCKL EVSNFQQPYI VNRTFMLAKE ASLADNNTDV RLIGEKLFRG VSAKDQCYLM KQVLNFTLED VLLPQSDRFQ PYMQEVVPFL TKLSNQLSSC HISGDDQNIQ KNVRRLKETV KKLGESGEIK AIGELDLLFM SLRNACV

Assay Protocol

It is recommended to reconstitute the lyophilized pegylated mouse IL22 in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted with other aqueous solutions.

Endotoxin

< 0.1 ng/µg of protein (< 1EU/µg)

Purity

> 98.0% as determined by Gel filtration and SDS-PAGE gel.

Bioactivity

Recombinant murine IL-22 is biologically active when compared to standard using STAT3 phosphorylation assay in HepG cells. Its acrtivity in vitro is ~ 10% compared to the non-pegylated mIL22.

Length

147

Form

Lyophilized

Reconstitution

Water

Molecular Weight

36.0 kDa (monomer)

Host or Source

E. coli

N Terminal Sequence

ALPVN

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